概述

  • 产品名称Anti-CD45RA抗体[MEM-56]
    参阅全部 CD45RA 一抗
  • 描述
    小鼠单克隆抗体[MEM-56] to CD45RA
  • 特异性CD45RA antigen (human)
  • 经测试应用适用于: IHC-P, WB, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). (Thymocytes and T lymphocytes).

性能

应用

Our Abpromise guarantee covers the use of ab8217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
WB Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.
Flow Cyt Use a concentration of 1 - 4 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

靶标

  • 功能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • 疾病相关Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
    Multiple sclerosis
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域The first PTPase domain interacts with SKAP1.
  • 翻译后修饰Heavily N- and O-glycosylated.
  • 细胞定位Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • EC 3.1.3.48 antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • Loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lymphocyte antigen 5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • RT7 antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45RA antibody [MEM-56] 图像

  • Lane 1 : CD45 control antibody
    Lanes 2 - 3 : Anti-CD45RA antibody [MEM-56] (ab8217)

    Lane 1 : HUT-78 human cutaneous T cell lymphoma cell line.
    Lane 2 : sample of cell lysate prepared under non-reducing conditions
    Lane 3 : sample of cell lysate prepared under reducing conditions

Anti-CD45RA antibody [MEM-56] (ab8217)参考文献

This product has been referenced in:
  • Abdulamir AS  et al. Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma. BMC Immunol 9:73 (2008). ICC ; Human . Read more (PubMed: 19087256) »
  • Bazil V  et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). Read more (PubMed: 2534480) »

See all 3 Publications for this product

Product Wall

Thank you for your patience in this matter. In regards to ab30476 (CD45RA antibody [F8-11-13] (Biotin)), the originator does not know which epitope this antibody is recognizing. However, information available in the literature suggests that it reco...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"