Anti-CD45R抗体[RA3-6B2] (PE/Cy5®) (ab25525)


  • 产品名称Anti-CD45R抗体[RA3-6B2] (PE/Cy5®)
    参阅全部 CD45R 一抗
  • 描述
    大鼠单克隆抗体[RA3-6B2] to CD45R (PE/Cy5®)
  • 偶联物PE/Cy5®. Ex: 496nm, Em: 670nm
  • 特异性ab25525 is specific for the B220 isoform of CD45, Mr 240 kDa.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Abelson leukemia virus-induced pre-B tumor cells (Mouse)

  • 常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    In vivo administration of ab25525 has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice.



Our Abpromise guarantee covers the use of ab25525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.


  • 功能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 疾病相关Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域The first PTPase domain interacts with SKAP1.
  • 翻译后修饰Heavily N- and O-glycosylated.
  • 细胞定位Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • Glycoprotein antibody
    • GP180 antibody
    • L CA antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • Leukocyte common antigen precursor antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor type tyrosine protein phosphatase C antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45R antibody [RA3-6B2] (PE/Cy5®) (ab25525)参考文献

This product has been referenced in:
  • Olme CH  et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23806234) »
  • Asensi V  et al. In vivo treatment with anti B-220 monoclonal antibody affects T and B cell differentiation. Clin Exp Immunol 80:268-73 (1990). Read more (PubMed: 1694118) »

See all 4 Publications for this product

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I can confirm that ab25525 CD45R antibody [RA3-6B2] (PE/Cy5®) has been tested in flow cytometry and in human samples as described on the datasheet. it will be covered by the 6 month guarantee for this applicat...

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