概述

  • 产品名称Anti-CD45.1抗体[A20] (FITC)
    参阅全部 CD45.1 一抗
  • 描述
    小鼠单克隆抗体[A20] to CD45.1 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性This antibody recognizes all mouse leukocytes expressing the CD 45.1 allotype.
  • 经测试应用适用于: IHC-P, Flow Cyt, ICC/IF, IP, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明


    This antibody is useful as a marker for origin of donor and host cells.

性能

应用

Our Abpromise guarantee covers the use of ab24917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性CD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
  • 细胞定位Type I membrane protein.
  • 数据库链接
  • 别名
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • LY5 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Receptor type tyrosine protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45.1 antibody [A20] (FITC) 图像

  • Flow Cytometry analysis of SJL mouse splenocytes labeling CD45.1 with ab24917 at 1 μg/106 cells dilution (purple). Grey - Isotype Control, Mouse IgG2a-FITC.

  • SJL spleeen cells were stained with ab24917, following which small lymphocytes were gated and analyzed on a FACScanTM flow cytometer (BIDS, San Jose, CA).

Anti-CD45.1 antibody [A20] (FITC) (ab24917)参考文献

This product has been referenced in:
  • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Mouse . Read more (PubMed: 25936350) »
  • Salmond RJ  et al. The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals. Nat Immunol 15:875-83 (2014). Read more (PubMed: 25108421) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Rodent Block M (Biocare Medical) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution pH6.1 (DAKO)
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative Paraformaldehyde
Username

Mr. Eike Wegener

Verified customer

提交于 Sep 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"