Anti-CD45抗体[MEM-28] (PerCP) (ab65952)

概述

  • 产品名称
    Anti-CD45抗体[MEM-28] (PerCP)
    参阅全部 CD45 一抗
  • 描述
    小鼠单克隆抗体[MEM-28] to CD45 (PerCP)
  • 偶联物
    PerCP. Ex: 482nm, Em: 675nm
  • 特异性
    ab65952 reacts with all alternative forms of human CD45 antigen.
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation;Human thymocytes and T lymphocytes.

  • 阳性对照
    • Flow Cyt: Human blood cells.
  • 常规说明

    The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

应用

Our Abpromise guarantee covers the use of ab65952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

Use 10µl of neat solution for 106 cells or 100µl of whole blood

ab118658 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 疾病相关
    Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 序列相似性
    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域
    The first PTPase domain interacts with SKAP1.
  • 翻译后修饰
    Heavily N- and O-glycosylated.
  • 细胞定位
    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • Protein tyrosine phosphatase, receptor type C antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45 antibody [MEM-28] (PerCP) (ab65952)参考文献

This product has been referenced in:
  • Gholizadeh-Ghaleh Aziz S  et al. Isolation, Characterization, Cryopreservation of Human Amniotic Stem Cells and Differentiation to Osteogenic and Adipogenic Cells. PLoS One 11:e0158281 (2016). Flow Cyt ; Human . Read more (PubMed: 27434028) »
  • Sadallah S  et al. Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells. J Immunol 186:6543-52 (2011). Flow Cyt ; Human . Read more (PubMed: 21525379) »

See all 2 Publications for this product

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Thank you very much for your phone call yesterday and for your patience while I have been in touch with the lab.

Ab65952 was tested in flow cytometry with a primary incubation time of 20-30 minutes at room temperature in the dark.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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