Anti-CD45抗体[MEM-28] (Allophycocyanin) (ab28106)


  • 产品名称Anti-CD45抗体[MEM-28] (Allophycocyanin)
    参阅全部 CD45 一抗
  • 描述
    小鼠单克隆抗体[MEM-28] to CD45 (Allophycocyanin)
  • 偶联物Allophycocyanin. Ex: 645nm, Em: 660nm
  • 特异性ab28106 reacts with all alternative forms of human CD45 antigen
  • 经测试应用适用于: Flow Cyt, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Thymocytes and T lymphocytes. (Human)

  • 常规说明

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.


Our Abpromise guarantee covers the use of ab28106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.


  • 功能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 疾病相关Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域The first PTPase domain interacts with SKAP1.
  • 翻译后修饰Heavily N- and O-glycosylated.
  • 细胞定位Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45 antibody [MEM-28] (Allophycocyanin) 图像

  • ab28106 staining the CD45 in Human PBMCs by Flow Cytometry. Cells were prepared by Ficoll-Hypaque isolation of PBMCs from buffy coat. The sample was incubated with the primary antibody (1/25 in PBS + 2% Human serum and 1mM EDTA) for 25 minutes at 4°C.

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Anti-CD45 antibody [MEM-28] (Allophycocyanin) (ab28106)参考文献

This product has been referenced in:
  • Horejsí V  et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha) 34:23-34 (1988). Read more (PubMed: 2968928) »

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The concentration of the next lot of ab28106 that would come into stock is 55 ug/ml.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll-Hypaque isolation of PBMCs from buffy coat
Sample buffer: PBS
Permeabilization No
Gating Strategy lymphocytes

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提交于 Mar 25 2013