Anti-CD45抗体[IBL-3/16] (ab23910)
Key features and details
- Rat monoclonal [IBL-3/16] to CD45
- Suitable for: IHC-Fr, IP, ICC/IF, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
概述
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产品名称
Anti-CD45抗体[IBL-3/16]
参阅全部 CD45 一抗 -
描述
大鼠单克隆抗体[IBL-3/16] to CD45 -
宿主
Rat -
经测试应用
适用于: IHC-Fr, IP, ICC/IF, Flow Cytmore details
不适用于: IHC-P,IHC-R or WB -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Tissue, cells or virus corresponding to Mouse CD45. Purified B cells from mouse lymph nodes.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯化说明
Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
IBL-3/16 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23910于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
Use 50ul of the suggested working dilution to label 10^5 cells in 100ul. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Use 50ul of the suggested working dilution to label 10^5 cells in 100ul. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
疾病相关
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
结构域
The first PTPase domain interacts with SKAP1. -
翻译后修饰
Heavily N- and O-glycosylated. -
细胞定位
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 19264 Mouse
- SwissProt: P06800 Mouse
- Unigene: 391573 Mouse
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别名
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab23910 被引用在 17 文献中.
- Mamillapalli R et al. Therapeutic role of uterine-derived stem cells in acute kidney injury. Stem Cell Res Ther 13:107 (2022). PubMed: 35279204
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Soni S et al. Microvesicle-Mediated Communication Within the Alveolar Space: Mechanisms of Uptake by Epithelial Cells and Alveolar Macrophages. Front Immunol 13:853769 (2022). PubMed: 35572508
- Hardy RA et al. Role of age and neuroinflammation in the mechanism of cognitive deficits in sickle cell disease. Exp Biol Med (Maywood) 246:106-120 (2021). PubMed: 32962408
- Jiang D et al. Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin. Nat Commun 11:5653 (2020). PubMed: 33159076