Anti-CD45抗体[I3/2.3] (Phycoerythrin -Texas Red®) (ab51482)

概述

  • 产品名称Anti-CD45抗体[I3/2.3] (Phycoerythrin -Texas Red®)
    参阅全部 CD45 一抗
  • 描述
    大鼠单克隆抗体[I3/2.3] to CD45 (Phycoerythrin -Texas Red®)
  • 偶联物Phycoerythrin -Texas Red®. Ex: 496nm, Em: 613nm
  • 特异性ab51482 is specific to CD45 (T200 or leukocyte common antigen/LCA, all isoforms).
  • 经测试应用适用于: RIA, Flow Cyt, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

应用

Our Abpromise guarantee covers the use of ab51482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
RIA Use at an assay dependent concentration. Quantification of CD45 molecules on cell surfaces.
Flow Cyt Use 0.2µg for 106 cells. ab98984-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • 疾病相关Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域The first PTPase domain interacts with SKAP1.
  • 翻译后修饰Heavily N- and O-glycosylated.
  • 细胞定位Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45 antibody [I3/2.3] (Phycoerythrin -Texas Red®) 图像

  • ab51482 staining CD45 in Mouse speen cells by Flow Cytometry.  The splenocytes were dispersed with 70µm strainer and erythrolyzed in PBS. The sample was incubated with the primary antibody (undiluted) for 30 minutes at 0°C.

    Gateing Strategy: FSC, SSC.

     

    See Abreview

  • ab51482 staining the CD45 in Mouse blood by Flow Cytometry. Cells were prepared in EDTA blood and the sample buffer was EDTA buffer. The sample was incubated with the primary antibody (1/100 in PBS) for 30 minutes at 20°C.
    Gating Strategy: FR - SS.

    See Abreview

Anti-CD45 antibody [I3/2.3] (Phycoerythrin -Texas Red®) (ab51482)参考文献

This product has been referenced in:
  • Oerlemans MI  et al. Active Wnt signaling in response to cardiac injury. Basic Res Cardiol 105:631-41 (2010). Flow Cyt . Read more (PubMed: 20373104) »
  • Haidl ID  et al. Detection of restricted isoform expression and tyrosine phosphatase activity of CD45 in murine dendritic cells. Eur J Immunol 25:3370-4 (1995). Read more (PubMed: 8566025) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (spleen)
Specification spleen
Preparation Cell harvesting/tissue preparation method: splenocytes dispersed with 70µm strainer and erythrolyzed
Sample buffer: PBS
Permeabilization No
Gating Strategy FSC, SSC
Username

Abcam user community

Verified customer

提交于 Mar 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (blood)
Specification blood
Preparation Cell harvesting/tissue preparation method: EDTA bood
Sample buffer: EDTA buffer
Fixation optilyse
Permeabilization No
Gating Strategy FS - SS
Username

Abcam user community

Verified customer

提交于 Mar 12 2013

Thank you for contacting us. I am sorry this vial did not contain the correct amount of antibody. As requested, I have issued a free of charge replacement with the order number ***.

To check the status of the order please contact our Custome...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"