Anti-CD45抗体[I3/2.3] (ab25386)
Key features and details
- Rat monoclonal [I3/2.3] to CD45
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2b
概述
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产品名称
Anti-CD45抗体[I3/2.3]
参阅全部 CD45 一抗 -
描述
大鼠单克隆抗体[I3/2.3] to CD45 -
宿主
Rat -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to CD45. T1M1 (Thy-1─c) cells
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表位
ab25386 recognizes a framework epitope present on all CD45 isoforms. -
阳性对照
- BALB/c spleen cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on (28 March 2019). Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
I3/2.3 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25386于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use at an assay dependent concentration.
See Haidl et al. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use at an assay dependent concentration. See Haidl et al. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
疾病相关
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
结构域
The first PTPase domain interacts with SKAP1. -
翻译后修饰
Heavily N- and O-glycosylated. -
细胞定位
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 19264 Mouse
- SwissProt: P06800 Mouse
- Unigene: 391573 Mouse
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别名
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
图片
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Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD45 with ab25386 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2b-PE was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB, followed by Mouse Anti-Rat IgG2b-PE.
This image was generated using the ascites version of the product.
数据表及文件
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SDS download
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Datasheet download
文献 (44)
ab25386 被引用在 44 文献中.
- Helms EJ et al. Mesenchymal Lineage Heterogeneity Underlies Nonredundant Functions of Pancreatic Cancer-Associated Fibroblasts. Cancer Discov 12:484-501 (2022). PubMed: 34548310
- Zhou X et al. SM22α-lineage niche cells regulate intramembranous bone regeneration via PDGFRβ-triggered hydrogen sulfide production. Cell Rep 39:110750 (2022). PubMed: 35508129
- Perdigoto AL et al. Immune cells and their inflammatory mediators modify β cells and cause checkpoint inhibitor-induced diabetes. JCI Insight 7:N/A (2022). PubMed: 35925682
- Rahrmann EP et al. The NALCN channel regulates metastasis and nonmalignant cell dissemination. Nat Genet 54:1827-1838 (2022). PubMed: 36175792
- Jin Y et al. Pathogenesis Study of Enterovirus 71 Using a Novel Human SCARB2 Knock-In Mouse Model. mSphere 6:N/A (2021). PubMed: 33692197