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Tissue/ cell preparation of Human T lymphocytes
Clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.
This antibody clone is manufactured by Abcam.
Our Abpromise guarantee covers the use of ab30470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|Flow Cyt||1/10 - 1/50. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||1/500 - 1/1000.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Ab30470 staining CD45 in Jurkat cells. The cells were fixed with 4% formaldehyde (10min), then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab30470 at 5ug/ml (shown in green) and ab206369, Rabbit monoclonal to beta Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). This was followed by an incubation at room temperature for 1h with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, at 1ug/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 80% methanol (5 min).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"