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Tissue/ cell preparation (Human): Non T, non B ALL cell line (REH).
Our Abpromise guarantee covers the use of ab33533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at 2 µg/mg of lysate.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 147 kDa.|
ab33533 staining human blood mononuclear cells by ICC/IF. Cells were PFA fixed and blocked in 2.5% BSA for 30 minutes at 20°C prior to incubation with the primary antibody, diluted 1/100, for 18 hours at 4°C. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"