The details of the immunogen for this antibody are not available.
ab25052 recognizes CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.2 allotype (e.g. A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). It is useful as a marker for origin of donor and host cells
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Primary antibody说明ab25052 recognizes CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.2 allotype (e.g. A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). It is useful as a marker for origin of donor and host cells
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
功能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
疾病相关Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. Contains 2 fibronectin type-III domains. Contains 2 tyrosine-protein phosphatase domains.
结构域The first PTPase domain interacts with SKAP1.
翻译后修饰Heavily N- and O-glycosylated.
细胞定位Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
Protein tyrosine phosphatase receptor type c polypeptide antibody
Protein tyrosine phosphatase, receptor type C antibody
protein tyrosine phosphatase, receptor type, C antibody
Protein tyrosine phosphatase, receptor type, c polypeptide antibody
Receptor-type tyrosine-protein phosphatase C antibody
T200 glycoprotein antibody
T200 leukocyte common antigen antibody
Anti-CD45 antibody [104-2] 图像
Inhibition Assay - Anti-CD45 antibody [104-2] (ab25052)Image from Antoine-Moussiaux N et al., PLoS One. 2009 May 29;4(5):e5728. doi: 10.1371/journal.pone.0005728.; Fig 11.; May 29, 2009, PLoS ONE 4(5): e5728.
Analysis of C57BL/6 mouse splenocyte necrosis after exposure to Trypanosoma evansi trypanosomes in the presence or absence of anti-CD45 antibodies. Splenocyte necrosis was blocked with ab25052 at 15 µg/5.105 cells.
C: Mock-exposed splenocytes
T: Splenocytes exposed to the trypanosomes after preconditioning with PBS
T CD45.1: Splenocytes exposed to the trypanosomes after preconditioning with ab25078
T CD45.2: Splenocytes exposed to the trypanosomes after preconditioning with ab25052
Anti-CD45 antibody [104-2] (ab25052)参考文献
This product has been referenced in:
Antoine-Moussiaux N et al. A non-cytosolic protein of Trypanosoma evansi induces CD45-dependent lymphocyte death. PLoS One4:e5728 (2009).
Read more (PubMed: 19478957) »