- 数据表
- 具体的参考文献 (3)
- 实验方案
概述
- 产品名称Anti-CD44v6抗体[VFF-18]
参阅全部 CD44v6 一抗 - 描述小鼠单克隆抗体[VFF-18] to CD44v6
- 特异性Specific for an epitope encoded by exon v6 on the variant portion of human CD44.
- 经测试应用适用于: WB, IHC-P, ICC, Flow Cyt, IHC-Frmore details
- 种属反应性与反应: Human
- 免疫原
Fusion protein corresponding to Human CD44v6. Glutathione S Transferase (GST) fusion protein corresponding to the variable domain v6 of human CD44.
Database link: P16070 - 表位Specific for an epitope encoded by exon v6 on the variant portion of human CD44.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: PBS -
Concentration information loading... - 纯度Protein G purified
- 克隆单克隆
- 克隆编号VFF-18
- 同种型IgG1
- 研究领域
相关产品
- Compatible Secondaries
- Isotype control
- Related Products
应用
Our Abpromise guarantee covers the use of ab78960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 91 kDa (predicted molecular weight: 82 kDa). | |
| IHC-P | Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| ICC | Use at an assay dependent concentration. Use on cell smears at an assay dependent dilution. |
|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-Fr | Use a concentration of 1 - 2 µg/ml. Use at a concentration of 1 - 2 µg/ml. Use acetone fixation and the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedures. |
靶标
- 功能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
- 组织特异性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
- 序列相似性Contains 1 Link domain.
- 结构域The lectin-like LINK domain is responsible for hyaluronan binding.
- 翻译后修饰Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. - 细胞定位Membrane. Colocalizes with actin in membrane protrusions at wounding edges.
- Information by UniProt
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数据库链接
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
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别名
- CD44 antibody
- CD44 antigen antibody
- CD44 molecule (Indian blood group) antibody
see all
Anti-CD44v6 antibody [VFF-18] 图像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [VFF-18] (ab78960)](http://www.abcam.cn/ps/products/78/ab78960/Images/CD44v6-Primary-antibodies-ab78960-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [VFF-18] (ab78960)IHC image of ab78960 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78960, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-CD44v6 antibody [VFF-18] (ab78960)](http://www.abcam.cn/ps/products/78/ab78960/Images/CD44v6-Primary-antibodies-ab78960-2.jpg)
Western blot - Anti-CD44v6 antibody [VFF-18] (ab78960)All lanes : Anti-CD44v6 antibody [VFF-18] (ab78960) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 82 kDa
Observed band size : 91 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa,40 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutesCD44v6 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
![Flow Cytometry - Anti-CD44v6 antibody [VFF-18] (ab78960)](http://www.abcam.cn/ps/products/78/ab78960/Images/ab78960-4-ab78960FC.jpg)
Flow Cytometry - Anti-CD44v6 antibody [VFF-18] (ab78960)Overlay histogram showing THP1 cells stained with ab78960 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78960, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunohistochemistry (Frozen sections) - Anti-CD44v6 antibody [VFF-18] (ab78960)](http://www.abcam.cn/ps/products/78/ab78960/Images/CD44v6-Primary-antibodies-ab78960-7.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD44v6 antibody [VFF-18] (ab78960)Image courtesy of Dr Rene Villadsen by Abreview.ab78960 staining CD44v6 in human breast cancer tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in methanol and then blocked using 10% serum for 5 minutes at 25°C. Samples were then incubated with ab78960 at a 1/500 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.
Anti-CD44v6 antibody [VFF-18] (ab78960)参考文献
This product has been referenced in:
- Okada T et al. Coexpression of EpCAM, CD44 Variant Isoforms and Claudin-7 in Anaplastic Thyroid Carcinoma. PLoS One 9:e94487 (2014). IHC-P ; Human . Read more (PubMed: 24727741) »
- Alamo P et al. Subcutaneous preconditioning increases invasion and metastatic dissemination in mouse colorectal cancer models. Dis Model Mech 7:387-96 (2014). IHC-P . Read more (PubMed: 24487410) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"