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Fusion protein corresponding to Human CD44v6. Glutathione S Transferase (GST) fusion protein corresponding to the variable domain v6 of human CD44.
Database link: P16070
This product was changed from ascites to tissue culture supernatant on 15th August 2017. The highest lot of ascites still in stock on 15th August 2017 is GR295277-3 . Lot numbers higher than GR295277-3 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab78960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 91 kDa (predicted molecular weight: 82 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
CD44v6 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab78960 (Ascites) staining CD44v6 in human breast cancer tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol and then blocked using 10% serum for 5 minutes at 25°C. Samples were then incubated with ab78960 at a 1/500 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.
Overlay histogram showing THP1 cells stained with ab78960 (Ascites) (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78960, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Immunocytochemical analysis of methanol fixed cultured human breast epithelial organoids labeling CD44v6 with ab78960 (Ascites) at 1/500 dilution. UltraVision ONE HRP Polymer was used as the secondary antibody. 10% serum was used as the blocking agent.
IHC image of ab78960 (Ascites) staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78960, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"