Anti-CD44 (phospho S706)抗体(ab63389)

概述

  • 产品名称Anti-CD44 (phospho S706)抗体
    参阅全部 CD44 一抗
  • 描述
    兔多克隆抗体to CD44 (phospho S706)
  • 特异性Detects endogenous CD44 only when phosphorylated at serine 706.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human CD44 around the phosphorylation site of serine 706.

  • 阳性对照
    • NIH/3T3 cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab63389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 82 kDa).

靶标

  • 功能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 组织特异性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 序列相似性Contains 1 Link domain.
  • 结构域The lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻译后修饰Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    see all

Anti-CD44 (phospho S706) antibody 图像

  • All lanes : Anti-CD44 (phospho S706) antibody (ab63389) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 5mins)
    Lane 2 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 5mins) with Phospho peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

Anti-CD44 (phospho S706) antibody (ab63389)参考文献

This product has been referenced in:
  • Wang YT  et al. An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9:5582-97 (2010). WB ; Human . Read more (PubMed: 20815410) »

See 1 Publication for this product

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After reading the protocol I am sure that the whole plate is coated with the antibody as all the reagents are designed to be sufficient for 12 stripes. As mentioned in the protocol, the 32 wells are just an examp...

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