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Tissue/ cell preparation: Cos 7 cells (African Green Monkey).
Our Abpromise guarantee covers the use of ab58754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 20µl for 106 cells.
(Good for 10^6 cells in suspension or for 100 µl of whole blood.)
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"