Anti-CD44抗体[MEM-263] (Allophycocyanin) (ab81424)

概述

  • 产品名称Anti-CD44抗体[MEM-263] (Allophycocyanin)
    参阅全部 CD44 一抗
  • 描述
    小鼠单克隆抗体[MEM-263] to CD44 (Allophycocyanin)
  • 偶联物Allophycocyanin. Ex: 645nm, Em: 660nm
  • 特异性Reacts with the extracellular (N-terminal) domain of CD44.
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Dog, Human, Pig
  • 免疫原

    COS-7 cells

性能

应用

Our Abpromise guarantee covers the use of ab81424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • 组织特异性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • 序列相似性Contains 1 Link domain.
  • 结构域The lectin-like LINK domain is responsible for hyaluronan binding.
  • 翻译后修饰Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    see all

Anti-CD44 antibody [MEM-263] (Allophycocyanin) 图像

  • ab81424 staining CD44 in human MDA-MB-231 cells by Immunocytochemistry/ Immunofluorescence.
    Tissue was fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 3% FBS for 30 minutes at 22°C and then incubated with ab81424 at a 1/200 dilution for 1 hour at 22°C.

    See Abreview

Anti-CD44 antibody [MEM-263] (Allophycocyanin) (ab81424)参考文献

ab81424 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. We do have products that are conjugated to match your needs, Ab81424 Anti-CD44 antibody [MEM-263] is conjugated with APC. Ab58754 Anti-CD44 antibody [MEM-263] (Phycoerythrin). I've included links to each below: http://www.a...

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Thank you very much for your excellent feedback. I added the discount code and published your Abreview, so that the discount code is now active and you can get a free primary antibody from us. Your account has also been updated with 220 Abpoints. To ch...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MDA-MB-231)
Specification MDA-MB-231
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton-X100
Blocking step FBS as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Jan 03 2012

Thanks for your reply and for the information. Ab77219 and ab81424 have been tested in flow cytometry and are guaranteed to work in FC with human cells. You are correct, they have not been tested in ICC/IF, and it is possible that they will wor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"