Anti-CD44抗体[KM201] (ab25340)
Key features and details
- Rat monoclonal [KM201] to CD44
- Suitable for: Flow Cyt, IHC-FoFr
- Reacts with: Mouse
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD44抗体[KM201]
参阅全部 CD44 一抗 -
描述
大鼠单克隆抗体[KM201] to CD44 -
宿主
Rat -
经测试应用
适用于: Flow Cyt, IHC-FoFrmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD44.
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表位
ab25340 reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. -
阳性对照
- Flow Cyt: BALB/c mouse bone marrow cells.
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常规说明
ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
KM201 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25340于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-FoFr |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt
Use at an assay dependent concentration. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-FoFr
Use at an assay dependent concentration. |
靶标
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功能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
组织特异性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
序列相似性
Contains 1 Link domain. -
结构域
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻译后修饰
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12505 Mouse
- SwissProt: P15379 Mouse
- Unigene: 423621 Mouse
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别名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab25340 被引用在 28 文献中.
- Sari RP et al. The Effect of Anadara granosa Shell's-Stichopus hermanni Scaffold on CD44 and IL-10 Expression to Decrease Osteoclasts in Socket Healing. Eur J Dent 15:228-235 (2021). PubMed: 33511601
- Shan Z et al. Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment. Elife 10:N/A (2021). PubMed: 34110284
- Toda H et al. Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence. Nat Commun 11:2761 (2020). PubMed: 32487994
- Fang S et al. The role of pulmonary mesenchymal cells in airway epithelium regeneration during injury repair. Stem Cell Res Ther 10:366 (2019). PubMed: 31791401
- Wang D et al. BMP14 induces tenogenic differentiation of bone marrow mesenchymal stem cells in vitro. Exp Ther Med 16:1165-1174 (2018). PubMed: 30116367