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Purified human T cells
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Our Abpromise guarantee covers the use of ab46793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200. See Abreview.|
|Flow Cyt||1/10 - 1/100. ab103534-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Flow Cytometry analysis of Human peripheral blood lymphocytes labeling CD44 with ab46793 at 10 μL/106 cells (purple). Grey - Isotype Control, Mouse IgG2a-PE/CY7.
Overlay histogram showing peripheral blood lymphocytes stained with ab46793 (red line). The cells were incubated with the antibody (ab46793, 1/100) for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) with secondary antibody DyLight® 649 goat anti-mouse IgG (H&L) (ab96882) at 1/200 dilution for 30 min at 4ºC. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"