RabMAb

Anti-CD44抗体[EPR1013Y] (ab51037)

概述

性能

应用

Our Abpromise guarantee covers the use of ab51037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • 组织特异性Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • 序列相似性Contains 1 Link domain.
    • 结构域The lectin-like LINK domain is responsible for hyaluronan binding.
    • 翻译后修饰Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      see all

    Anti-CD44 antibody [EPR1013Y] 图像

    • ab51037 (1:100) showing positive staining in human Breast carcinoma tissue.

    • ab51037 used at a 1/200 dilution (red) to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Pgoat anti-rabbit IgG (Alexa Fluor® 488) polyclonal used at a 1/2000 dilution. Isotype control (black): Rabbit monoclonal IgG (ab172730). Unlabelled control (Blue): Cell without incubation with primary antibody and secondary antibody.

    • Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • All lanes : Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/1000 dilution

      Lane 1 : MCF-7 whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : MDA-MB-231 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 82 kDa
      Observed band size : 81 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer 5% NFDM/TBST

      The expression of CD44 in MCF-7 is low (PMID: 25635866; PMID: 26005723). Jurkat does not express CD44 (PMID: 24127558).

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). The sample was counter stained with hematoxylin.

    • Anti-CD44 antibody [EPR1013Y] (ab51037) at 1/5000 dilution + TF-1 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size : 82 kDa
      Observed band size : 82 kDa
    • ab51037 (1:100) showing positive staining in human Glioma tissue.

    • ab51037 (1:100) showing positive staining in human Thyroid gland carcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CD44 antibody [EPR1013Y] (ab51037)参考文献

    This product has been referenced in:
    • Saad A  et al. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia. Stem Cell Res Ther 7:128 (2016). Flow Cyt ; Human . Read more (PubMed: 27612459) »
    • Choi SH  et al. Dual-function synthetic peptide derived from BMP4 for highly efficient tumor targeting and antiangiogenesis. Int J Nanomedicine 11:4643-4656 (2016). IHC-P ; Human . Read more (PubMed: 27695323) »

    See all 29 Publications for this product

    Product Wall


    According to SwissProt, the immunogen range indicates it can detect most isoforms, excluding only isoform 2 and 19.

    http://www.uniprot.org/uniprot/P16070


    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jun 03 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hela)
    Gel Running Conditions Non-reduced Denaturing (7.5% SDS-PAGE)
    Loading amount 40 µg
    Specification Hela
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 10 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
    Sample Mouse Tissue sections (Mouse 15.5dpc)
    Specification Mouse 15.5dpc
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 May 14 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Fixation Paraformaldehyde
    Permeabilization No
    Sample Mouse Cell (differentiating ES)
    Specification differentiating ES
    Gating Strategy live cells
    Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
    Sample buffer: enzyme free
    Username

    Abcam user community

    Verified customer

    提交于 Jul 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Skin)
    Specification Skin
    Fixative Formalin
    Antigen retrieval step Other
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 12 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MB-MDA-231)
    Loading amount 20 µg
    Specification MB-MDA-231
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Ms. Zahra Abdalla

    Verified customer

    提交于 Dec 19 2012

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody in mouse.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarante...

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    Vielen Dank für Ihren Anruf.

    Leider handelt es sich bei diesem Produkt um einen Antikörper, der sich noch in dem ursprünglichen Zellkultur- Überstand befindet: dies macht eine Messung der Antikörperkonzentration aufgru...

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    Thank you for contacting us.
    As discussed over the phone, could you please send me the lot numbers?
    Thank you.

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