概述
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产品名称CD43 overexpression 293T裂解液(全细胞)
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描述CD43 overexpression裂解液
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常规说明ab94089 is a 293T cell transfected lysate in which Human CD43 has been transiently over-expressed using a pCMV-CD43 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用适用于: WBmore details
性能
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形式Liquid
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存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
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存储溶液pH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... -
纯度Whole Cell Lysate
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研究领域
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背景Function: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Tissue specificity: Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. PTM: Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
应用
Our Abpromise guarantee covers the use of ab94089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. |
CD43 overexpression 293T lysate (whole cell) 图像
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SDS-PAGE - CD43 293T Transfected Lysate - (positive control) (ab94089)ab94089 at 15µg/lane on an SDS-PAGE gel. -
Western blot - CD43 293T Transfected Lysate - (positive control) (ab94089)All lanes : Anti-CD43 antibody (ab68421) at 1/500 dilution
Lane 1 :CD43 overexpression 293T lysate (whole cell) (ab94089)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
CD43 overexpression 293T lysate (whole cell) (ab94089)参考文献
ab94089 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"