Anti-CD43抗体[MEM-59] (ab9088)
Key features and details
- Mouse monoclonal [MEM-59] to CD43
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD43抗体[MEM-59]
参阅全部 CD43 一抗 -
描述
小鼠单克隆抗体[MEM-59] to CD43 -
宿主
Mouse -
特异性
Recognizes neuraminidase-sensitive epitope on human CD43 molecule (Leukosialin), which is involved in lymphocyte activation. -
经测试应用
适用于: Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD43. Human T lymphocytes
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阳性对照
- IHC-P: Human spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from TCS. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
MEM-59 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
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IHC-P |
Use a concentration of 10 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 1 - 5 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
组织特异性
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
翻译后修饰
Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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别名
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
图片
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Flow cytometric analysis of Human Peripheral Blood cells labelling CD43 with ab9088 at 2 ug/ml showing separation of human CD43 positive lymphocytes (red-filled) from human CD43 negative blood debris (black-dashed).
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Immunohistochemical analysis of Paraffin-embedded sections Human Spleen tissue labelling CD43 with ab9088.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab9088 被引用在 7 文献中.
- Martin-Jaular L et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J 40:e105492 (2021). PubMed: 33709510
- Wisnovsky S et al. Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33495350
- Cermak L et al. Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem 277:7955-61 (2002). PubMed: 11773067
- Alvarado M et al. MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol 25:1051-5 (1995). PubMed: 7537669
- Deckert M et al. CD59 molecule: a second ligand for CD2 in T cell adhesion. Eur J Immunol 22:2943-7 (1992). PubMed: 1385156
- Stefanova I et al. Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol 26:153-61 (1989). PubMed: 2918859
- Stefanova I et al. Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha) 34:255-65 (1988). PubMed: 2467832