概述

  • 产品名称Anti-CD43抗体
    参阅全部 CD43 一抗
  • 描述
    小鼠单克隆抗体to CD43
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human CD43 protein with tag (AAH12350, 1 a.a. ~ 401 a.a)

  • 阳性对照
    • CD43 transfected 293T lysate.

性能

应用

Our Abpromise guarantee covers the use of ab68421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).

靶标

  • 功能One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
  • 组织特异性Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • 翻译后修饰Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 43 antibody
    • CD43 antibody
    • CD43 antigen antibody
    • Galactoglycoprotein antibody
    • GALGP antibody
    • GPL 115 antibody
    • GPL115 antibody
    • Human gene for sialophorin antibody
    • Leucocyte sialoglycoprotein antibody
    • LEUK_HUMAN antibody
    • Leukocyte large sialoglycoprotein antibody
    • Leukocyte sialoglycoprotein antibody
    • Leukosialin antibody
    • LSN antibody
    • Ly-48 antibody
    • sialophorin (gpL115, leukosialin, CD43) antibody
    • Sialophorin antibody
    • Spn antibody
    see all

Anti-CD43 antibody 图像

  • Anti-CD43 antibody (ab68421) at 1/500 dilution + immunising protein

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 2500

    Predicted band size : 40 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CD43 antibody (ab68421) at 1/500 dilution

    Lane 1 : CD43 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-CD43 antibody (ab68421)参考文献

ab68421 has not yet been referenced specifically in any publications.

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