Anti-CD42d抗体(ab65017)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD42d
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human CD42d.

    (Peptide available as ab74425.)

  • 阳性对照
    • This antibody gave a positive signal in the following Lysates: EBS E14 TG2A Day 8 Whole Cell, Raji Whole Cell, EBS E14 TG2A Day 0 Whole Cell, EBS E14 TG2A Day 5 Whole Cell

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 61 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能
    The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis.
  • 组织特异性
    Platelets and megakaryocytes.
  • 序列相似性
    Contains 13 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • 翻译后修饰
    The N-terminus is blocked.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 42d antibody
    • CD42d antibody
    • CD42d antigen antibody
    • Glycoprotein 5 antibody
    • Glycoprotein V antibody
    • Glycoprotein V platelet antibody
    • GlycoproteinV antibody
    • Gp 5 antibody
    • GP V antibody
    • GP5 antibody
    • GPV antibody
    • GPV_HUMAN antibody
    • Platelet glycoprotein V antibody
    • Platelet glycoprotein V precursor antibody
    see all

图片

  • All lanes : Anti-CD42d antibody (ab65017) at 1 µg/ml

    Lane 1 : EBS E14 TG2A Day 8 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : EBS E14 TG2A Day 0 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 4 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 82 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    CD42d has a predicted molecular weight of 61 kDa but contains a number of potential glycosylation sites (SwissProt). We believe that the observed 81 kDa band correlates to the glycosylated form of the uncleaved protein. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

文献

ab65017 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Rat Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (Bio-rad Mini-PROTEAN® TGX™ Precast Gels)
Loading amount
20 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Feb 28 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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