Anti-CD41抗体[MWReg30] (ab33661)


  • 产品名称Anti-CD41抗体[MWReg30]
    参阅全部 CD41 一抗
  • 描述
    大鼠单克隆抗体[MWReg30] to CD41
  • 特异性ab33661 recognizes integrin alpha IIb subunit CD41
  • 经测试应用适用于: IHC-P, Flow Cyt, IHC-Fr, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明The azide free version is ab94960.

    Clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo.



Our Abpromise guarantee covers the use of ab33661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 20525356
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.

ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/300.
IP Use at an assay dependent concentration.


  • 功能Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
  • 组织特异性Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
  • 疾病相关Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • 序列相似性Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • antigen CD41 antibody
    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD41 antibody
    • CD41B antibody
    • form 2 antibody
    • GP2B antibody
    • GPalpha IIb antibody
    • GPIIb antibody
    • GT antibody
    • GTA antibody
    • HPA3 antibody
    • Integrin alpha 2b antibody
    • Integrin alpha IIb antibody
    • Integrin alpha-IIb light chain antibody
    • Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) antibody
    • ITA2B_HUMAN antibody
    • Itga2b antibody
    • ITGAB antibody
    • platelet fibrinogen receptor, alpha subunit antibody
    • platelet glycoprotein IIb of IIb/IIIa complex antibody
    • Platelet membrane glycoprotein IIb antibody
    • platelet specific antigen BAK antibody
    • PPP1R93 antibody
    see all

Anti-CD41 antibody [MWReg30] 图像

  • ab33661 staining CD41 in mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 9 hours at 4°C. A Biotin-conjugated goat anti-rat IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • IHC-Fr image of CD41 staining on mouse lung sections using ab33661 (1:300). The the sections came from PFA fixed and snap frzen tissue and 10 micron sections were cut using a cryostat. The sections were blocked with 1% BSA at 21°C for 10 mins. The sections were then incubated with ab33661 for 2 hours at 21°C. Goat Polyclonal to Rat IgG conjugated to Biotin was used at 1:200.

    See Abreview

Anti-CD41 antibody [MWReg30] (ab33661)参考文献

This product has been referenced in:
  • Belnoue E  et al. Homing and adhesion patterns determine the cellular composition of the bone marrow plasma cell niche. J Immunol 188:1283-91 (2012). Mouse . Read more (PubMed: 22262758) »
  • Cervi D  et al. Micronutrients attenuate progression of prostate cancer by elevating the endogenous inhibitor of angiogenesis, platelet factor-4. BMC Cancer 10:258 (2010). IHC-P ; Mouse . Read more (PubMed: 20525356) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Lung)
Permeabilization No
Specification Lung
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde

Mr. Simon Cleary

Verified customer

提交于 Jun 18 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

提交于 Nov 05 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human primary megakaryocytes)
Permeabilization Yes - 0.1% Triton-X100
Specification Human primary megakaryocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Aug 19 2016

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification liver, hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Feb 05 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (spleen)
Specification spleen
Permeabilization No
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Mar 26 2014

Thank you for contacting us. I am afraid we don’t have specific protocols for each antibody. Each staining procedure might be optimised for every assay, depending on the application tested, the species, the samples, etc... We have protocols in our webs...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spleen 6um)
Specification spleen 6um
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 15°C

Dr. Lynda Coughlan

Verified customer

提交于 Jan 04 2011