概述

  • 产品名称
    Anti-CD40L抗体[EP462E]
    参阅全部 CD40L 一抗
  • 描述
    兔单克隆抗体[EP462E] to CD40L
  • 特异性
    This antibody reacts with CD40L.
  • 经测试应用
    适用于: IHC-P, WBmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues of human CD40L.

  • 阳性对照
    • WB: MOLT-4 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab52750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 27882144
WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能
      Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
    • 组织特异性
      Specifically expressed on activated CD4+ T-lymphocytes.
    • 疾病相关
      Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
    • 序列相似性
      Belongs to the tumor necrosis factor family.
    • 翻译后修饰
      The soluble form derives from the membrane form by proteolytic processing.
      N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
      Not O-glycosylated.
    • 细胞定位
      Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 40L antibody
      • CD154 antibody
      • CD40 antigen ligand antibody
      • CD40 ligand antibody
      • CD40 ligand, soluble form antibody
      • CD40-L antibody
      • CD40L antibody
      • CD40L_HUMAN antibody
      • CD40LG antibody
      • gp39 antibody
      • hCD40L antibody
      • HIGM1 antibody
      • IGM antibody
      • IMD3 antibody
      • T B cell activating molecule antibody
      • T BAM antibody
      • T-cell antigen Gp39 antibody
      • TNF-related activation protein antibody
      • TNFSF5 antibody
      • TrAP antibody
      • Tumor necrosis factor (ligand) superfamily member 5 antibody
      • Tumor necrosis factor ligand superfamily member 5 antibody
      see all

    Anti-CD40L antibody [EP462E] 图像

    • Anti-CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 36 kDa
      Observed band size : 36 kDa

    Anti-CD40L antibody [EP462E] (ab52750)参考文献

    This product has been referenced in:
    • Sun KT  et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). Flow Cyt ; Human . Read more (PubMed: 28303002) »
    • Wang X  et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med 12:3242-3248 (2016). IHC-P ; Rabbit . Read more (PubMed: 27882144) »

    See all 2 Publications for this product

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