重组Anti-TRAP/CD40L抗体[EP462E] (ab52750)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP462E] to TRAP/CD40L
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-TRAP/CD40L抗体[EP462E]
参阅全部 TRAP/CD40L 一抗 -
描述
兔单克隆抗体[EP462E] to TRAP/CD40L -
宿主
Rabbit -
特异性
This antibody reacts with TRAP/CD40L. Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC. -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: MOLT-4 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP462E -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52750于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
靶标
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功能
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
组织特异性
Specifically expressed on activated CD4+ T-lymphocytes. -
疾病相关
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 959 Human
- Entrez Gene: 21947 Mouse
- Omim: 300386 Human
- SwissProt: P29965 Human
- SwissProt: P27548 Mouse
- Unigene: 592244 Human
- Unigene: 4861 Mouse
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别名
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
图片
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All lanes : Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1 µg/ml
Lane 1 : Human lymph node tissue lysate
Lane 2 : Mouse lymph node tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaBlocking and diluting buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 90 seconds
Lane 2: 20 seconds
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Anti-TRAP/CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab52750 被引用在 8 文献中.
- Yang B et al. 3-hydroxy butyrate dehydrogenase 2 deficiency aggravates systemic lupus erythematosus progression in a mouse model by promoting CD40 ligand demethylation. Bioengineered 13:2685-2695 (2022). PubMed: 35001849
- Zhang X et al. MicroRNA-455-3p regulates proliferation and osteoclast differentiation of RAW264.7 cells by targeting PTEN. BMC Musculoskelet Disord 23:340 (2022). PubMed: 35397519
- Ma M et al. Baohuoside I Inhibits Osteoclastogenesis and Protects Against Ovariectomy-Induced Bone Loss. Front Pharmacol 13:874952 (2022). PubMed: 35571086
- Yajun W et al. Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone. Front Pharmacol 12:723988 (2021). PubMed: 34658862
- Chen H et al. MYC-mediated miR-320a affects receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast formation by regulating phosphatase and tensin homolog (PTEN). Bioengineered 12:12677-12687 (2021). PubMed: 34933640
- Vigil FA et al. Prevention of brain damage after traumatic brain injury by pharmacological enhancement of KCNQ (Kv7, "M-type") K+ currents in neurons. J Cereb Blood Flow Metab 40:1256-1273 (2020). PubMed: 31272312
- Sun KT et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). Flow Cyt ; Human . PubMed: 28303002
- Wang X et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med 12:3242-3248 (2016). IHC-P ; Rabbit . PubMed: 27882144