Anti-CD40L抗体(ab65854)

概述

  • 产品名称Anti-CD40L抗体
    参阅全部 CD40L 一抗
  • 描述
    兔多克隆抗体to CD40L
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    A synthetic peptide corresponding to a sequence at the N terminal of Human CD40L, different from the relative mouse sequence by two amino acids.

  • 阳性对照
    • IHC-P: Mammary cancer sections Wb: Rat spleen tissue

性能

应用

Our Abpromise guarantee covers the use of ab65854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • 组织特异性Specifically expressed on activated CD4+ T-lymphocytes.
  • 疾病相关Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 40L antibody
    • CD154 antibody
    • CD40 antigen ligand antibody
    • CD40 ligand antibody
    • CD40 ligand, soluble form antibody
    • CD40-L antibody
    • CD40L antibody
    • CD40L_HUMAN antibody
    • CD40LG antibody
    • gp39 antibody
    • hCD40L antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • T B cell activating molecule antibody
    • T BAM antibody
    • T-cell antigen Gp39 antibody
    • TNF-related activation protein antibody
    • TNFSF5 antibody
    • TrAP antibody
    • Tumor necrosis factor (ligand) superfamily member 5 antibody
    • Tumor necrosis factor ligand superfamily member 5 antibody
    see all

Anti-CD40L antibody 图像

  • ab65854, at 2µg/ml, staining CD40L in the cytoplasm of paraffin embedded mammary cancer tissue by Immunohistochemistry. DAB chromogenic reaction.
  • Anti-CD40L antibody (ab65854) at 2 µg/ml + Rat spleen

    Predicted band size : 29 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa,38 kDa,39 kDa. We are unsure as to the identity of these extra bands.


  • Predicted band size : 29 kDa

Anti-CD40L antibody (ab65854)参考文献

ab65854 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (MCF-7)
Specification MCF-7
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Sample Human Tissue sections (Breast carcinoma)
Specification Breast carcinoma
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 16 2013

Thank you once again for your follow up email.

I am pleased to let you know I have now heard back from the originator with a copy of the general IHC-P protocol that they use for testing. Please note this will be a guideline only and may requ...

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Thank you for contacting us.
The antibody ab104686 is not in clear solution it is indeed in whole antiserum. We would recommend vortexing the antibody for a while and then after centrifuging using it as normal product. If in any case the antibody ...

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Thank you for taking the time to complete our questionnaire. I am sorry to hear you have had difficulty submitting and sending the information to us. I can confirm we have now received the questionnaire and the images from you. I appreciate your patien...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forIHC-...

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Thank you for contacting Abcam regarding our CD40 and CD40L antibodies. Below please find the requested information: ab2391 Rabbit Polyclonal to CD40L - Unfortunately the specific immunogen sequence is proprietary, however I can inform you that...

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Thank you for your enquiry. Please find the protocols as below: 1.Cover the entire surface of a clean microslide with APES or POLY-L-LYSINE. Incubate for 1 minute then rinse the microslide with water. Mount a tissue section (~5μm thick) with the tr...

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