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Our Abpromise guarantee covers the use of ab2391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/200. See Abreview|
|ELISA||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/100. Fix with acetone -20°C for 10 min (provides a better signal than unfixed tissue; PFA 4% abolishes the signal). Incubate primary for 24h at 4°C. Use amplification (indirect IF).|
ab2391 staining CD40L in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
Immunofluorescent analysis of paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells CD40L with ab2391 at 1/200 dilution, followed by Chicken Anti-Rabbit IgG H&L (Alexa Fluor® 488 secondary antibody at 1/750 dilution (green). WGA is detected with Alexa Fluor® 568 Wheat Germ Agglutinin (red). The nuclear counter stain is DAPI (blue).
ab2391 at 1/500 staining rat spleen tissue sections by IHC-P. The tissue was fomraldehyde fixed and blocked with serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor ® 488 conjugated goat anti-rabbit was used as the secondary.
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