概述

  • 产品名称Anti-CD40抗体
    参阅全部 CD40 一抗
  • 描述
    兔多克隆抗体to CD40
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CD40.

  • 阳性对照
    • This antibody gave a positive signal in Human Spleen and Thymus tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal spleen.

性能

应用

Our Abpromise guarantee covers the use of ab113701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 31 kDa).
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Receptor for TNFSF5/CD40LG.
  • 组织特异性B-cells and in primary carcinomas.
  • 疾病相关Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • 序列相似性Contains 4 TNFR-Cys repeats.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI326936 antibody
    • B cell associated molecule CD40 antibody
    • B cell surface antigen CD40 antibody
    • B cell-associated molecule antibody
    • B-cell surface antigen CD40 antibody
    • Bp50 antibody
    • CD 40 antibody
    • CD40 antibody
    • CD40 antigen (TNF receptor superfamily member 5) antibody
    • CD40 antigen antibody
    • CD40 molecule antibody
    • CD40 molecule, TNF receptor superfamily member 5 antibody
    • CD40 protein antibody
    • CD40 type II isoform antibody
    • CD40L receptor antibody
    • CDw40 antibody
    • GP39 antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • MGC9013 antibody
    • Nerve growth factor receptor related B lymphocyte activation molecule antibody
    • OTTHUMP00000031699 antibody
    • OTTHUMP00000031700 antibody
    • p50 antibody
    • T-BAM antibody
    • TBAM antibody
    • TNF receptor superfamily member 5 antibody
    • TNFRSF5 antibody
    • TNR5_HUMAN antibody
    • TRAP antibody
    • Tumor necrosis factor receptor superfamily , member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 precursor antibody
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
    see all

Anti-CD40 antibody 图像

  • All lanes : Anti-CD40 antibody (ab113701) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,41 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • IHC image of CD40 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113701, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CD40 antibody (ab113701)参考文献

ab113701 has not yet been referenced specifically in any publications.

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