Anti-CD40抗体(ab113701)
Key features and details
- Rabbit polyclonal to CD40
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD40抗体
参阅全部 CD40 一抗 -
描述
兔多克隆抗体to CD40 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Macaque monkey, Gorilla -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: U-2 OS and Raji cell lysates; Human spleen and thymus tissue lysates. IHC-FFPE: Human spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113701于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 31 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
靶标
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功能
Receptor for TNFSF5/CD40LG. -
组织特异性
B-cells and in primary carcinomas. -
疾病相关
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. -
序列相似性
Contains 4 TNFR-Cys repeats. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 958 Human
- Omim: 109535 Human
- SwissProt: P25942 Human
- Unigene: 472860 Human
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别名
- AI326936 antibody
- B cell associated molecule CD40 antibody
- B cell surface antigen CD40 antibody
see all
图片
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All lanes : Anti-CD40 antibody (ab113701) at 1 µg/ml
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : CD40 knockout U-2 OS cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab113701 observed at 45 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab113701 was shown to react with CD40 in wild-type U-2 OS cells in Western blot. Loss of signal was observed when CD40 knockout cell line ab262486 (knockout cell lysate ab263923) was used. Wild-type and CD40 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab113701 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-CD40 antibody (ab113701) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 41 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
CD40 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of CD40 is 31 kDa (SwissProt), however we expect to observe a banding pattern around 43 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
IHC image of CD40 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113701, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113701 尚未被引用在任何文献中。