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Our Abpromise guarantee covers the use of ab34276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||1/100 - 1/200. Use 10µl of this concentration to label 106 cells in 100µl.
ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Immunohistochemistry (Frozen sections) analysis of human palatine tonsil tissue labelling CD4 (red) with ab34276 at 1/50. A Cy3-conjugated donkey anti-rat IgG was used as the secondary antibody. Green - SLURP-1, Blue - Nuclei (DAPI).