Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Staining of CD4 in normal Human peripheral blood cells with Mouse IgG1 Isotype Control PE-Cy5 (open histogram) or ab95512 at 0.25 µg/test (filled histogram). Cells in the lymphocyte gate were used for analysis.