特异性The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen.
MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions. It is negative in Western blotting even with non-reduced samples of cell lysates.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Flow Cyt: Use at an assay dependent dilution.
Notes: Although it has not been tested rigorously, data suggest that this antibody is a low-affinity antibody: its binding to T cells increases at elevated temperature; monovalent Fab fragments essentially do not bind to T cells.
FuncS: Use at an assay dependent dilution.
This antibody blocks binding of HIV gp120 to CD4 molecule and it also strongly
inhibits CD4-MHC Class II interactions.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.