Anti-CD4抗体[MEM115] (ab46674)

概述

  • 产品名称Anti-CD4抗体[MEM115]
    参阅全部 CD4 一抗
  • 描述
    小鼠单克隆抗体[MEM115] to CD4
  • 特异性The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen. MEM-115 blocks binding of HIV gp120 to CD4 molecule and it also strongly inhibits CD4-MHC Class II interactions. It is negative in Western blotting even with non-reduced samples of cell lysates.
  • 经测试应用适用于: IP, Functional Studies, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human thymocytes and T lymphocytes.

  • 常规说明Preserved by filter sterilization.

性能

应用

Our Abpromise guarantee covers the use of ab46674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
Functional Studies
Flow Cyt
  • 应用说明Flow Cyt: Use at an assay dependent dilution.
    Notes: Although it has not been tested rigorously, data suggest that this antibody is a low-affinity antibody: its binding to T cells increases at elevated temperature; monovalent Fab fragments essentially do not bind to T cells.

    FuncS: Use at an assay dependent dilution.
    This antibody blocks binding of HIV gp120 to CD4 molecule and it also strongly
    inhibits CD4-MHC Class II interactions.

    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • 序列相似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • 细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [MEM115] (ab46674)参考文献

    ab46674 has not yet been referenced specifically in any publications.

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