Anti-CD4抗体[MEM-241] (PerCP) (ab65951)

概述

  • 产品名称
    Anti-CD4抗体[MEM-241] (PerCP)
    参阅全部 CD4 一抗
  • 描述
    小鼠单克隆抗体[MEM-241] to CD4 (PerCP)
  • 偶联物
    PerCP. Ex: 482nm, Em: 675nm
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human, Cynomolgus monkey
  • 免疫原

    Two N terminal domains of human CD4 fused to human IgG1 Fc

  • 常规说明

    The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

性能

应用

Our Abpromise guarantee covers the use of ab65951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. ab118658-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 序列相似性
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 细胞定位
    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    图片

    • Flow cytometry analysis of Human whole blood after red blod cell lysis, staining CD4 with ab65951. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

      Gating Strategy: Live lymphocytes

      See Abreview

    • Flow cytometry analysis of Cynomolgus monkey whole blood after red blod cell lysis, staining CD4 with ab65951. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

      Gating Strategy: Live lymphocytes

      See Abreview

    文献

    This product has been referenced in:
    • Xue L  et al. Prostaglandin D2 activates group 2 innate lymphoid cells through chemoattractant receptor-homologous molecule expressed on TH2 cells. J Allergy Clin Immunol 133:1184-94 (2014). Flow Cyt ; Human . Read more (PubMed: 24388011) »
    • Bastien JP  et al. Photodepletion differentially affects CD4+ Tregs versus CD4+ effector T cells from patients with chronic graft-versus-host disease. Blood 116:4859-69 (2010). Flow Cyt ; Human . Read more (PubMed: 20798236) »

    See all 2 Publications for this product

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Username

    Abcam user community

    Verified customer

    提交于 Jan 09 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: isolated from spleen
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Username

    Abcam user community

    Verified customer

    提交于 Jan 02 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Cynomolgus Monkey Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Username

    Abcam user community

    Verified customer

    提交于 Jan 02 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive lymphocytes
    Username

    Abcam user community

    Verified customer

    提交于 Jan 02 2013

    Thank you for contacting us. The lab has confirmed that this antibody is indeed specific to CD4. We will guarantee it not to react with any human IgG1 that may be present in your experiments. I hope this helps, please let me know if you need any additi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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