Anti-CD4抗体[GK1.5] (Phycoerythrin) (ab25496)

概述

  • 产品名称Anti-CD4抗体[GK1.5] (Phycoerythrin)
    参阅全部 CD4 一抗
  • 描述
    大鼠单克隆抗体[GK1.5] to CD4 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性ab25496 recognises CD4/L3T4.
  • 经测试应用适用于: Flow Cyt, IP, Functional Studies, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.

  • 常规说明


    This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

性能

应用

Our Abpromise guarantee covers the use of ab25496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IP
Functional Studies
IHC-Fr
  • 应用说明Flow Cyt: Use 0.2µg for 106 cells.
    FuncS (In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function; Fractionation of CD4+ cells): Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • 序列相似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • 细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody [GK1.5] (Phycoerythrin) 图像

    • Flow cytometry analysis of BALB/c mouse splenocytes stained with ab25496 and rat anti-mouse CD8α-PE.

    • his FACS image shows two colour staining of BALB/c mouse mesentereic lymph node cells stained with ab25496 and anti-CD8-FITC.  Cells were then gated on small lymphocytes and analyzed by flow cytometry.

    Anti-CD4 antibody [GK1.5] (Phycoerythrin) (ab25496)参考文献

    ab25496 has not yet been referenced specifically in any publications.

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