Anti-CD4抗体[GK1.5] (Cy5.5 ®) (ab41467)

概述

  • 产品名称Anti-CD4抗体[GK1.5] (Cy5.5 ®)
    参阅全部 CD4 一抗
  • 描述
    大鼠单克隆抗体[GK1.5] to CD4 (Cy5.5 ®)
  • 偶联物Cy5.5 ®. Ex: 678nm, Em: 703nm
  • 特异性CD4/L3T4, 52 kDa
  • 经测试应用适用于: IHC-Fr, Flow Cyt, IP, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Cloned mouse CTL line V4

  • 常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4+ cells. The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

性能

应用

Our Abpromise guarantee covers the use of ab41467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.

靶标

  • 功能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 序列相似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [GK1.5] (Cy5.5 ®) 图像

  • Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD4 with ab41467 at 0.1 μg/106 cells dilution. A Rat Anti-Mouse CD8α-FITC was used as the secondary antibody.

Anti-CD4 antibody [GK1.5] (Cy5.5 ®) (ab41467)参考文献

This product has been referenced in:
  • Bluestone JA  et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol Rev 120:5-33 (1991). Read more (PubMed: 1650760) »
  • Havran WL & Fitch FW Effects of anti-Lyt-2 and anti-L3T4 monoclonal antibodies on the function of cytotoxic T lymphocyte/helper T lymphocyte hybrid T cell clones. J Immunol 141:1808-12 (1988). Read more (PubMed: 2459190) »

See all 8 Publications for this product

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To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated ...

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Thank you for your enquiry and your patience. Unfortunately, I've confirmed that this product has not been tested specifically for IHC-P, and only for IHC-Fr. I have updated the datasheet to include this information and I'm sorry for any inconvenien...

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