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Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.
This antibody also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Our Abpromise guarantee covers the use of ab25532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab136586-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration.
In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function; Fractionation of CD4+ cells.
Flow cytometry analysis of BALB/c mouse splenocytes stained with ab25532 and rat anti-mouse CD8α-FITC.
ab25532 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"