Anti-CD4抗体[GK1.5] (Biotin) (ab19641)

概述

  • 产品名称Anti-CD4抗体[GK1.5] (Biotin)
    参阅全部 CD4 一抗
  • 描述
    大鼠单克隆抗体[GK1.5] to CD4 (Biotin)
  • 偶联物Biotin
  • 特异性Recognizes a determinant designated L3T4 present on mouse T-cell surface with an apparent molecular mass of 52kDa. The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
  • 经测试应用适用于: Flow Cyt, ICC/IF, IPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Lyt-2-negative T-cell clone 243/2.5

性能

应用

Our Abpromise guarantee covers the use of ab19641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 序列相似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [GK1.5] (Biotin) 图像

  • C57Bl/6 splenocytes stained with 0.25µg ab19641 (solid line) or 0.25µg biotin-conjugated rat IgG2b isotype control (dashed line), followed by Streptavidin PE.

Anti-CD4 antibody [GK1.5] (Biotin) (ab19641)参考文献

ab19641 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Tissue sections (Mouse oral tumor cells (MOC-2 obtained from Uppalu)
Specification Mouse oral tumor cells (MOC-2 obtained from Uppalu
Permeabilization No
Fixative Acetone
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提交于 Mar 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"