概述

  • 产品名称Anti-CD4抗体[GK1.5]
    参阅全部 CD4 一抗
  • 描述
    大鼠单克隆抗体[GK1.5] to CD4
  • 特异性ab25475 recognises CD4/L3T4, Mr 52 kDa.
  • 经测试应用适用于: Functional Studies, Flow Cyt, IP, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.

  • 常规说明

    This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.

性能

应用

Our Abpromise guarantee covers the use of ab25475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration. PubMed: 6415170(In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function and Fractionation of CD4+ cells) Use at an assay dependent dilution. For details see: Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1. See also PubMed: 6415170. For details see: Kruisbeek, A. M., et al.
Flow Cyt Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21345609

靶标

  • 功能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 序列相似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [GK1.5] 图像

  • ab25475 staining CD4 (red) in mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 12 hours at 4°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    Blue: DAPI.

    See Abreview

  • Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD4 with ab25475 at 1 μg/106 cells dilution (purple). A Mouse Anti-Rat IgG2b-PE was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB followed by Mouse Anti-Rat IgG2b-PE.

Anti-CD4 antibody [GK1.5] (ab25475)参考文献

This product has been referenced in:
  • Yoon JH  et al. Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. Nat Commun 6:7600 (2015). IHC ; Mouse . Read more (PubMed: 26194464) »
  • Kim MC  et al. Supplementation of Influenza Split Vaccines with Conserved M2 Ectodomains Overcomes Strain Specificity and Provides Long-term Cross Protection. Mol Ther N/A:N/A (2014). Read more (PubMed: 24590045) »

See all 9 Publications for this product

Product Wall


I am very pleased to hear you would like to accept our offer and test ab25475 in IHC-P. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (Liver, Spleen, Lung)
Specification Liver, Spleen, Lung
Permeabilization No
Fixative no fixation
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Abcam user community

Verified customer

提交于 Aug 15 2013

I can confirm that these two antibodies ab25478 and ab25475 are tested externally in functional studies.

There are publications where these clones have been tested in this application. I have attached thse for your review and hope this will ...

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Our products of animal origin are derived from animals that have not given evidence of any disease, mentioned in list A of the Office of International des Epizooties (OIE). With that being said, our products are no...

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I am very pleased to hear you would like to accept our offer and test ab25475 inIHC-P. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIHC-P and include this code in the &ld...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Formaldehyde
Permeabilization Yes - 0.05%TritonX-100/PBS
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 12 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (tumor mouse)
Specification tumor mouse
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Miss. Silke Vorwald

Verified customer

提交于 Sep 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse skeletal muscle)
Specification mouse skeletal muscle
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"