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Tissue/ cell preparation (Mouse) - cloned mouse CTL line V4.
This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Our Abpromise guarantee covers the use of ab25475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. PubMed: 6415170(In vivo and in vitro depletion of CD4+ cells; Inhibition of T helper function and Fractionation of CD4+ cells) Use at an assay dependent dilution. For details see: Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1. See also PubMed: 6415170. For details see: Kruisbeek, A. M., et al.|
|Flow Cyt||Use at an assay dependent concentration.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21345609|
ab25475 staining CD4 (red) in mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS) for 12 hours at 4°C. A Cy3®-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD4 with ab25475 at 1 μg/106 cells dilution (purple). A Mouse Anti-Rat IgG2b-PE was used as the secondary antibody. Grey - Isotype Control, Rat IgG2b-UNLB followed by Mouse Anti-Rat IgG2b-PE.
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