Anti-CD4抗体[B-A1] (FITC) (ab59474)

概述

  • 产品名称Anti-CD4抗体[B-A1] (FITC)
    参阅全部 CD4 一抗
  • 描述
    小鼠单克隆抗体[B-A1] to CD4 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性Recognises a 55 kDa protein
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human, Cynomolgus monkey
  • 免疫原

    PHA activated T cells

性能

应用

Our Abpromise guarantee covers the use of ab59474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use 10µl for 106 cells or 100µl of blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • 序列相似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • 细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • 数据库链接
      • 别名
        • CD 4 antibody
        • CD4 (L3T4) antibody
        • CD4 antibody
        • CD4 antigen (p55) antibody
        • CD4 antigen antibody
        • CD4 molecule antibody
        • CD4 receptor antibody
        • CD4+ Lymphocyte deficiency, included antibody
        • CD4_HUMAN antibody
        • CD4mut antibody
        • L3T4 antibody
        • Leu3 antibody
        • Ly-4 antibody
        • Lymphocyte antigen CD4 antibody
        • MGC165891 antibody
        • OTTHUMP00000238897 antibody
        • p55 antibody
        • T cell antigen T4 antibody
        • T cell antigen T4/LEU3 antibody
        • T cell differentiation antigen L3T4 antibody
        • T cell OKT4 deficiency, included antibody
        • T cell surface antigen T4/Leu 3 antibody
        • T cell surface antigen T4/Leu3 antibody
        • T cell surface glycoprotein CD4 antibody
        • T-cell surface antigen T4/Leu-3 antibody
        • T-cell surface glycoprotein CD4 antibody
        • W3/25 antibody
        • W3/25 antigen antibody
        see all

      Anti-CD4 antibody [B-A1] (FITC) 图像

      • A typical staining pattern with the B-A1 monoclonal antibody of lymphocytes.
      • Flow cytometry analysis of Cynomolgus Monkey whole blood after red blod cell lysis, staining CD4 with ab59474. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

        Gating Strategy: Live lymphocytes

        See Abreview

      Anti-CD4 antibody [B-A1] (FITC) (ab59474)参考文献

      ab59474 has not yet been referenced specifically in any publications.

      Product Wall

      Abcam has not validated the combination of species/application used in this Abreview.
      Application Flow Cytometry
      Sample Rat Cell (Splenocytes)
      Specification Splenocytes
      Preparation Cell harvesting/tissue preparation method: isolated from spleen
      Sample buffer: PBS/BSA 1%
      Permeabilization No
      Gating Strategy alive lymphocytes
      Username

      Abcam user community

      Verified customer

      提交于 Jan 10 2013



      500 uL of the antibody is provided per 50-test vial.

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application Flow Cytometry
      Sample Human Cell (from buffy coat)
      Specification from buffy coat
      Preparation Cell harvesting/tissue preparation method: after ficoll extraction
      Sample buffer: PBS/BSA 1%
      Fixation none
      Permeabilization No
      Gating Strategy alive lymphocytes
      Username

      Abcam user community

      Verified customer

      提交于 Jan 04 2013

      Abcam has not validated the combination of species/application used in this Abreview.
      Application Flow Cytometry
      Sample Mouse Cell (Splenocytes)
      Specification Splenocytes
      Preparation Cell harvesting/tissue preparation method: isolated from spleen
      Sample buffer: PBS/BSA 1%
      Permeabilization No
      Gating Strategy alive lymphocytes
      Username

      Abcam user community

      Verified customer

      提交于 Jan 02 2013

      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application Flow Cytometry
      Sample Cynomolgus Monkey Cell (from whole blood)
      Specification from whole blood
      Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
      Sample buffer: PBS/BSA 1%
      Fixation Paraformaldehyde
      Permeabilization No
      Gating Strategy alive lymphocytes
      Username

      Abcam user community

      Verified customer

      提交于 Jan 02 2013

      Thank you for contacting Abcam.
      All of the antibodies that you mentioned and all the antibodies in our catalogue are guaranteed for 6 months under our Abpromise. In terms of stability, they should all have be the same, regardless of the BSA concen...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"