Anti-CD4抗体- Aminoterminal end (ab70951)

概述

  • 产品名称Anti-CD4抗体- Aminoterminal end
    参阅全部 CD4 一抗
  • 描述
    兔多克隆抗体to CD4 - Aminoterminal end
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human CD4 aa 58-86 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 920

  • 阳性对照
    • Transfected/ non-transfected 293 cell lysates and Hela cell line lysates.

性能

应用

Our Abpromise guarantee covers the use of ab70951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-P
  • 应用说明ELISA: 1/1000.
    IHC-P: 1/10 - 1/50.
    WB: 1/50 - 1/100. Detects a band of approximately 51kDa (Hela cell line lysates) and 60 kDa (transfected 293 cell lysates). (predicted molecular weight: 51 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • 序列相似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • 细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    Anti-CD4 antibody - Aminoterminal end 图像

    • Anti-CD4 antibody - Aminoterminal end (ab70951) at 1/60 dilution + Hela cell line lysates at 35 µg

      Predicted band size : 51 kDa
      Observed band size : 51 kDa
    • All lanes : Anti-CD4 antibody - Aminoterminal end (ab70951) at 1/50 dilution

      Lane 1 : Non-transfected 293 cell lysates
      Lane 2 : CD4 transfected 293 cell lysates

      Lysates/proteins at 2 µg per lane.


      Predicted band size : 51 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • ab70951 at 1/50 dilution staining CD4- Aminoterminal end in human Lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

    Anti-CD4 antibody - Aminoterminal end (ab70951)参考文献

    ab70951 has not yet been referenced specifically in any publications.

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