翻译后修饰Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
细胞定位Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Peripheral blood mononuclear cells were isolated from
heparinized pig blood on Ficoll-Hypaque density gradients and double-stained with ab25354 at 0.2ug/106 cells and mouse anti-pig CD8a-FITC. Lymphocytes were gated andanalyzed on a FACScan• flow cytometer (BDIS, San Jose, CA).