概述

  • 产品名称
    Anti-CD4抗体[10C12]
    参阅全部 CD4 一抗
  • 描述
    小鼠单克隆抗体[10C12] to CD4
  • 经测试应用
    适用于: ELISA, WB, ICC/IF, Blockingmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Cat, Dog, Pig, Chimpanzee, Monkey
  • 免疫原

    Synthetic peptide: LS EKKTCQCPHR FQKTCSPI, corresponding to C terminal amino acids 439-458 of Human CD4.

  • 表位
    This antibody recognizes an epitope within amino acids 414-433 of the cytoplasmic tail region of human CD4.
  • 阳性对照
    • Jurkat and H9 cell lysates for ELISA, and CEM cells on glass slides for IF.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab133487 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab25804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 20929345
Blocking Use at an assay dependent concentration. PubMed: 22745469

靶标

  • 功能
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • 序列相似性
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • 细胞定位
    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

文献

This product has been referenced in:
  • Atanackovic D  et al. Role of interleukin 16 in multiple myeloma. J Natl Cancer Inst 104:1005-20 (2012). Blocking ; Human . Read more (PubMed: 22745469) »
  • McCarty WJ  et al. The biophysical mechanisms of altered hyaluronan concentration in synovial fluid after anterior cruciate ligament transection. Arthritis Rheum 64:3993-4003 (2012). Read more (PubMed: 22933328) »

See all 4 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Permeabilization
No
Specification
small intestine
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Hsiao-Ying chiu

Verified customer

提交于 Dec 22 2014

Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

Es tut mir leid zu hören, dass unsere Vorschläge in diesem Fall nicht zu einer Verbesserung Ihrer Ergebnisse geführt haben. Ich weiß, dass Sie ...

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Thank you for contacting Abcam and for your interest in our products.

Regarding the CD4 antibody ab25804, based on a BLAST of the immunogen, there is no homology with rat, therefore I would not recommend this antibody to use with rat samples...

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Thank you for your email.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab846.
To check th...

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Thank you for your reply.
For PPAR alpha, I would recommend ab27478 as an isotype control.
http://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html

As for the CD4 antibody, your protocol seems to be fine. The immunogen sequence is l...

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Thank you for contacting us.
To answer your questions:
1. Yes, a negative control can be ommiting the primary. Other options could be using an isotype control antibody that compares with the priamry, or the blocking peptide. There are various...

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Thank you for your inquiry. Unfortunately, we do not offer an antibody that is tested and guaranteed for rabbit and IHC-P. We do not know if ab25804 is suitable for IHC-P. I would like to offer you a testing discount for this, but testing two unknowns ...

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Thank you for calling Abcam. I have continued to look through our catalogue for antibodies for CD4 and CD19, that are guaranteed to work in ICC/IF on mouse tissue. I have not found anything that is guaranteed to work in both your requirements, the best...

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Thank you for calling Abcam on Friday. For CD4, I have found the following set Protein: ab111463, fragment from 29-390. Antibodies: ab67480, aa7****                      ab51312, aa******* I know that the antibodies bind very close to each other, but t...

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Yes, but none that are raised against an intracellular domain. For instance, ab13438 is raised against an intact cell preparation. The clone, Q4120, blocks the receptor, confirming that the epitope it recognizes is extracellular. http://www.abcam.com/i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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