RabMAb

Anti-CD3D抗体[EP4426] (ab109531)

概述

  • 产品名称Anti-CD3D抗体[EP4426]
    参阅全部 CD3D 一抗
  • 描述
    兔单克隆抗体[EP4426] to CD3D
  • 经测试应用适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD3D (C terminal).

  • 阳性对照
    • Jurkat, HuT 78 and Human thymus lysates; Human tonsil tissue.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab109531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能The CD3 complex mediates signal transduction.
  • 疾病相关Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 序列相似性Contains 1 ITAM domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 形式.
  • 别名
    • CD3d antibody
    • CD3D_HUMAN antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody

Anti-CD3D antibody [EP4426] 图像

  • ab109531, at 1/100 dilution, staining paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • ab109531 staining CD3 in Jurkat cells by Flow Cytometry. Cells were fixed in paraformaldehyde and permeabilized in saponin. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 4°C. A phycoerythrin-conjugated Goat anti-rabbit IgG (1/100) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-CD3D antibody [EP4426] (ab109531) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HuT 78 cell lysate
    Lane 3 : Human thymus tissue lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 19 kDa

Anti-CD3D antibody [EP4426] (ab109531)参考文献

This product has been referenced in:
  • Alunno A  et al. Telocytes in minor salivary glands of primary Sjögren's syndrome: association with the extent of inflammation and ectopic lymphoid neogenesis. J Cell Mol Med 19:1689-96 (2015). IHC-P ; Human . Read more (PubMed: 25753463) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Sample Human Cell (Jurkat (T lymphocytes))
Specification Jurkat (T lymphocytes)
Permeabilization Yes - SAPONIN
Username

Ms. Beatriz Garcillan

Verified customer

提交于 Oct 15 2013

Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"