RabMAb

Anti-CD39抗体[EPR3678(2)] (ab108248)

概述

  • 产品名称Anti-CD39抗体[EPR3678(2)]
    参阅全部 CD39 一抗
  • 描述
    兔单克隆抗体[EPR3678(2)] to CD39
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human CD39.

  • 阳性对照
    • Human placenta and IM-9 cell lysates
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
    • 组织特异性Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only.
    • 序列相似性Belongs to the GDA1/CD39 NTPase family.
    • 翻译后修饰The N-terminus is blocked.
      Palmitoylated in the N-terminal part.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 形式There are 3 isoforms produced by alternative splicing.
    • 别名
      • ATPDase antibody
      • CD 39 antibody
      • CD39 antibody
      • CD39 antigen antibody
      • DKFZp686D194 antibody
      • DKFZp686I093 antibody
      • Ecto apyrase antibody
      • Ecto ATP diphosphohydrolase antibody
      • Ecto-apyrase antibody
      • Ecto-ATP diphosphohydrolase 1 antibody
      • Ecto-ATPase 1 antibody
      • Ecto-ATPDase 1 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 1 antibody
      • ENTP1_HUMAN antibody
      • ENTPD 1 antibody
      • ENTPD1 antibody
      • FLJ40921 antibody
      • FLJ40959 antibody
      • Lymphoid cell activation antigen antibody
      • NTPDase 1 antibody
      • NTPDase1 antibody
      • SPG64 antibody
      see all

    Anti-CD39 antibody [EPR3678(2)] 图像

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : Rat hippocampus
      Lane 2 : Rat pancreas

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70,74 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : Mouse placenta
      Lane 2 : Mouse urinary bladder

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

      The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.

    • Lane 1 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/100 dilution (purified)
      Lane 2 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : HUVEC whole cell lysate
      Lane 2 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70,74 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

      Ref: PMID: 18693757

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : IM-9 cell lysate


      Predicted band size : 58 kDa

    Anti-CD39 antibody [EPR3678(2)] (ab108248)参考文献

    This product has been referenced in:
    • Shah D  et al. Extracellular ATP mediates the late phase of neutrophil recruitment to the lung in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol 306:L152-61 (2014). WB . Read more (PubMed: 24285266) »
    • Traini M  et al. Sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is a novel nucleotide phosphodiesterase regulated by cholesterol in human macrophages. J Biol Chem 289:32895-913 (2014). Read more (PubMed: 25288789) »

    See all 3 Publications for this product

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