重组
RabMAb

Anti-CD39抗体[EPR3678(2)] (ab108248)

概述

  • 产品名称
    Anti-CD39抗体[EPR3678(2)]
    参阅全部 CD39 一抗
  • 描述
    兔单克隆抗体[EPR3678(2)] to CD39
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human CD39 aa 350-450. The exact sequence is proprietary.

  • 阳性对照
    • WB: Human placenta, rat hippocampus, rat pancreas, mouse placenta and mouse urinary bladder tissue lysates; IM-9 and HUVEC cell lysates.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能
      In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
    • 组织特异性
      Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only.
    • 序列相似性
      Belongs to the GDA1/CD39 NTPase family.
    • 翻译后修饰
      The N-terminus is blocked.
      Palmitoylated in the N-terminal part.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 形式
      There are 3 isoforms produced by alternative splicing.
    • 别名
      • ATPDase antibody
      • CD 39 antibody
      • CD39 antibody
      • CD39 antigen antibody
      • DKFZp686D194 antibody
      • DKFZp686I093 antibody
      • Ecto apyrase antibody
      • Ecto ATP diphosphohydrolase antibody
      • Ecto-apyrase antibody
      • Ecto-ATP diphosphohydrolase 1 antibody
      • Ecto-ATPase 1 antibody
      • Ecto-ATPDase 1 antibody
      • Ectonucleoside triphosphate diphosphohydrolase 1 antibody
      • ENTP1_HUMAN antibody
      • ENTPD 1 antibody
      • ENTPD1 antibody
      • FLJ40921 antibody
      • FLJ40959 antibody
      • Lymphoid cell activation antigen antibody
      • NTPDase 1 antibody
      • NTPDase1 antibody
      • SPG64 antibody
      see all

    Anti-CD39 antibody [EPR3678(2)] 图像

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : Rat hippocampus
      Lane 2 : Rat pancreas

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70,74 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : Mouse placenta
      Lane 2 : Mouse urinary bladder

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

      The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.

    • Lane 1 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/100 dilution (purified)
      Lane 2 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

      Lane 1 : HUVEC whole cell lysate
      Lane 2 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 58 kDa
      Observed band size : 70,74 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration 5% NFDM/TBST.

      Ref: PMID: 18693757

    • All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (unpurified)

      Lane 1 : Human placenta lysate
      Lane 2 : IM-9 cell lysate


      Predicted band size : 58 kDa

    实验方案

    Anti-CD39 antibody [EPR3678(2)] (ab108248)参考文献

    This product has been referenced in:
    • Villa-Bellosta R  et al. Novel phosphate-activated macrophages prevent ectopic calcification by increasing extracellular ATP and pyrophosphate. PLoS One 12:e0174998 (2017). Read more (PubMed: 28362852) »
    • Aldi S  et al. E-NTPDase1/CD39 modulates renin release from heart mast cells during ischemia/reperfusion: a novel cardioprotective role. FASEB J 29:61-9 (2015). Read more (PubMed: 25318477) »

    See all 5 Publications for this product

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