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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab24977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Cellular Activation||Use at an assay dependent concentration. Induction of B cell proliferation.|
|Flow Cyt||Use 0.2µg for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
Cells from BALB/c spleen were double-stained with ab24977 (at a concentration of 0.3 µg/106 cells) and rat anti-mouse CD19-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
ab24977 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"