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Our Abpromise guarantee covers the use of ab23680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 0.5 µg/ml. ab37322-Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody.|
|Blocking||Use a concentration of 2 µg/ml. (or 1/500).Note: ab23680 inhibits CD36 binding of ox-LDL (macrophages) and rod puter segments (retinal pigment epithelial cells) and prevents foam cell formation of macrophages.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22205027|
ab23680 staining the CD36 in RAW 264.7 cells by Flow Cytometry.
Immunofluorescence analysis of 3T3-L1 adipocytes, staining CD36 with ab23680.
Cells were fixed with paraformaldehyde, permeabilized with 0.01% saponin and blocked with 1% BSA. Samples were incubated with primary antibody overnight at 4°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"