重组Anti-CD36抗体[EPR6573] (ab133625)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6573] to CD36
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD36抗体[EPR6573]
参阅全部 CD36 一抗 -
描述
兔单克隆抗体[EPR6573] to CD36 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 57% homology with SCARB1. Cross-reactivity with this protein has not been confirmed experimentally. Expression levels of the target protein vary with sample type and some optimisation may be required.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Guinea pig -
免疫原
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阳性对照
- WB: HEK293, THP-1, HepG2, human adipose tissue and platelet lysates. IHC-P: human cardiac muscle and hepatocellular cancer tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6573 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133625于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (6) |
1/1000 - 1/10000. Detects a band of approximately 78-88 kDa (predicted molecular weight: 53 kDa).
For unpurified use at 1/100 - 1/1000. DS: For Lysate preparation protocol, please refer to the protocol book in the protocol section. Product runs at 75-85 kDa due to glycosylation. |
IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
We do not guarantee IHC-P for mouse species and did not test IHC-P on guinea pig tissues. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 78-88 kDa (predicted molecular weight: 53 kDa). For unpurified use at 1/100 - 1/1000. DS: For Lysate preparation protocol, please refer to the protocol book in the protocol section. Product runs at 75-85 kDa due to glycosylation. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. We do not guarantee IHC-P for mouse species and did not test IHC-P on guinea pig tissues. |
靶标
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功能
Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
(Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling. -
疾病相关
Platelet glycoprotein IV deficiency
Coronary heart disease 7 -
序列相似性
Belongs to the CD36 family. -
翻译后修饰
N-glycosylated and O-glycosylated with a ratio of 2:1.
Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783). -
细胞定位
Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis. - Information by UniProt
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数据库链接
- Entrez Gene: 948 Human
- Omim: 173510 Human
- SwissProt: P16671 Human
- Unigene: 120949 Human
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别名
- Adipocyte membrane protein antibody
- BDPLT10 antibody
- CD36 antibody
see all
图片
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All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA (Phorbol-12-myristate-13-acetate) for 72 hours whole cell lysates
Lane 3 : Human adipose lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 50 secondsThe expression level of CD36 varies in different samples, and it could be upregulated by treatments such as PMA and Porphyromonas gingivalis (PMID: 8576181 and 27234131).
Blocking and diluting buffer and concentration: 5% NFDM/TBST
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Ab133625 staining CD36 in paraffin embedded Human Hepatocellular cancer tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining on endothelial cells in human hepatocellular cancer.
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Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution + HEK293 (human embryonic kidney epithelial cell) transfected with His-tagged human CD36 (30aa-439aa) expression vector, whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer: 5% NFDM/TBST
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Ab133625 staining CD36 in paraffin embedded Human cardiac muscle tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining mainly on endothelial cells in human cardiac muscle.
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All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution
Lane 1 : Human Heart Tissue Lysate
Lane 2 : Human Adipose Tissue Lysate
Lane 3 : Human Platelet Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 53 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133625 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (111)
ab133625 被引用在 111 文献中.
- Zhu L et al. Faecal microbiota transplantation-mediated jejunal microbiota changes halt high-fat diet-induced obesity in mice via retarding intestinal fat absorption. Microb Biotechnol 15:337-352 (2022). PubMed: 34704376
- Wu K & Wang L Stomatin-knockdown effectively attenuates sepsis-induced oxidative stress and inflammation of alveolar epithelial cells by regulating CD36. Exp Ther Med 23:69 (2022). PubMed: 34934440
- Liu J et al. The role of CD36-Fabp4-PPARγ in skeletal muscle involves insulin resistance in intrauterine growth retardation mice with catch-up growth. BMC Endocr Disord 22:10 (2022). PubMed: 34983495
- Li H et al. Combined Use of Bicyclol and Berberine Alleviates Mouse Nonalcoholic Fatty Liver Disease. Front Pharmacol 13:843872 (2022). PubMed: 35250593
- Deng W et al. Farnesoid X Receptor Deficiency Induces Hepatic Lipid and Glucose Metabolism Disorder via Regulation of Pyruvate Dehydrogenase Kinase 4. Oxid Med Cell Longev 2022:3589525 (2022). PubMed: 35251469