Anti-CD36抗体(ab64014)

概述

  • 产品名称Anti-CD36抗体
    参阅全部 CD36 一抗
  • 描述
    兔多克隆抗体to CD36
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CD36.

    (Peptide available as ab99159.)

  • 阳性对照
    • This antibody gave a positive signal in Human Platelet Whole Cell Lysate, as well as Human Spleen and Adipose tissue lysates.

相关产品

应用

Our Abpromise guarantee covers the use of ab64014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).

Abcam recommends using BSA as the blocking agent.

靶标

  • 功能Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
    (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
  • 疾病相关Platelet glycoprotein IV deficiency
    Coronary heart disease 7
  • 序列相似性Belongs to the CD36 family.
  • 翻译后修饰N-glycosylated and O-glycosylated with a ratio of 2:1.
    Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
  • 细胞定位Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adipocyte membrane protein antibody
    • BDPLT10 antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
    • CD36 antigen antibody
    • CD36 molecule (thrombospondin receptor) antibody
    • CD36 molecule antibody
    • CD36_HUMAN antibody
    • CHDS7 antibody
    • Cluster determinant 36 antibody
    • Collagen receptor, platelet antibody
    • FAT antibody
    • Fatty acid translocase antibody
    • Fatty acid transport protein antibody
    • Glycoprotein IIIb antibody
    • GP IIIb antibody
    • GP3B antibody
    • GP4 antibody
    • GPIIIB antibody
    • GPIV antibody
    • Leukocyte differentiation antigen CD36 antibody
    • MGC108510 antibody
    • MGC91634 antibody
    • PAS 4 protein antibody
    • PAS IV antibody
    • PAS-4 antibody
    • PASIV antibody
    • Platelet collagen receptor antibody
    • Platelet glycoprotein 4 antibody
    • Platelet glycoprotein IV antibody
    • scarb3 antibody
    • Scavenger receptor class B member 3 antibody
    • Thrombospondin receptor antibody
    see all

Anti-CD36 antibody 图像

  • All lanes : Anti-CD36 antibody (ab64014) at 1 µg/ml

    Lane 1 : Human Platelet (Human adult normal cell line) Whole Cell Lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human adipose normal tissue lysate - total protein (ab28980)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • All lanes : Anti-CD36 antibody (ab64014) at 2 µg/ml (Blocked in 3% milk)

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate
    Lane 3 : Adult Mouse Brown Adipose Tissue Tissue Lysate
    Lane 4 : Adult Rat Brown Adipose Tissue Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 20 minutes

Anti-CD36 antibody (ab64014)参考文献

ab64014 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel)
Sample Mouse Tissue lysate - whole (Heart)
Specification Heart
Treatment Day 0 and day 7 post-MI
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Merry Lindsey

Verified customer

提交于 Sep 20 2013

Thank you for contacting us.
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Thank you for contacting us. I am sorry to hear that the antibody is giving problems. As we discussed yesterday over the phone, the following suggestions might help to find out if the bands you see in the 75-100 kDa range might be specific (as the ...

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Application Western blot
Sample Rat Tissue lysate - whole (adipose tissue)
Loading amount 30 µg
Specification adipose tissue
Gel Running Conditions Non-reduced Denaturing (10% acrylamide)
Blocking step BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Miss. Ilaria Ramaiola

Verified customer

提交于 Aug 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse blood film)
Specification Mouse blood film
Fixative Methanol
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 May 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"