概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD36
  • 经测试应用
    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide within Mouse CD36 aa 50-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    AFKNWVKTGTTVYRQFWIFDVQNPDDVAKNSSKIKVKQRGPYTYRVRYLA K


    Database link: Q08857

  • 阳性对照
    • Mouse heart lysate; Rat heart tissue; Mouse spleen cells.

性能

应用

Our Abpromise guarantee covers the use of ab216686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/1000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/500.
Flow Cyt 1/20 - 1/100.

靶标

  • 功能
    Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellulare calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
    (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.
  • 疾病相关
    Platelet glycoprotein IV deficiency
    Coronary heart disease 7
  • 序列相似性
    Belongs to the CD36 family.
  • 翻译后修饰
    N-glycosylated and O-glycosylated with a ratio of 2:1.
    Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome (PubMed:21610069, PubMed:18353783). Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids (PubMed:18353783).
  • 细胞定位
    Cell membrane. Membrane raft. Golgi apparatus. Apical cell membrane. Upon ligand-binding, internalized through dynamin-dependent endocytosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adipocyte membrane protein antibody
    • BDPLT10 antibody
    • CD36 antibody
    • CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody
    • CD36 antigen antibody
    • CD36 molecule (thrombospondin receptor) antibody
    • CD36 molecule antibody
    • CD36_HUMAN antibody
    • CHDS7 antibody
    • Cluster determinant 36 antibody
    • Collagen receptor, platelet antibody
    • FAT antibody
    • Fatty acid translocase antibody
    • Fatty acid transport protein antibody
    • Glycoprotein IIIb antibody
    • GP IIIb antibody
    • GP3B antibody
    • GP4 antibody
    • GPIIIB antibody
    • GPIV antibody
    • Leukocyte differentiation antigen CD36 antibody
    • MGC108510 antibody
    • MGC91634 antibody
    • PAS 4 protein antibody
    • PAS IV antibody
    • PAS-4 antibody
    • PASIV antibody
    • Platelet collagen receptor antibody
    • Platelet glycoprotein 4 antibody
    • Platelet glycoprotein IV antibody
    • scarb3 antibody
    • Scavenger receptor class B member 3 antibody
    • Thrombospondin receptor antibody
    see all

图片

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling CD36 with ab216686 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Anti-CD36 antibody (ab216686) at 1/300 dilution + Mouse heart lysate

    Secondary
    Goat anti-rabbit IgG (H+L), HRP conjugated at 1/500 dilution

    Predicted band size : 53 kDa
  • Flow cytometric analysis of Mouse spleen cells labeling CD36 with ab 216686 at 1/20 dilution for 30 minutes followed by incubation with a conjugated secondary antibody (green), compared to control cells (blue) secondary only (light blue) and isotype control (orange). 

文献

ab216686 has not yet been referenced specifically in any publications.

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