Anti-CD34抗体[QBEND / 10] (Biotin) (ab30375)

概述

  • 产品名称
    Anti-CD34抗体[QBEND / 10] (Biotin)
    参阅全部 CD34 一抗
  • 描述
    小鼠单克隆抗体[QBEND / 10] to CD34 (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Human, Cynomolgus monkey, Rhesus monkey
    不与反应: Rat, Sheep, Cow, Dog
  • 免疫原

    Human endothelial cell membrane vesicles.

  • 常规说明
    Each test is 10ul therefore the antibody volume supplied is 500ul.


    This antibody binds to the Class II epitope of CD34

性能

应用

Our Abpromise guarantee covers the use of ab30375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. in 100µl.



ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 组织特异性
    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 序列相似性
    Belongs to the CD34 family.
  • 发展阶段
    On early hematopoietic progenitor cells.
  • 翻译后修饰
    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

图片

  • ab30375 staining CD34 in human intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/50 in PBS/BSA and incubated with the sample for 12 hours at 4°C.

    See Abreview

文献

This product has been referenced in:
  • Sun JN  et al. Hepatoid adenocarcinoma of the lung without production of a-fetoprotein: A case report and review of the literature. Oncol Lett 12:189-194 (2016). IHC-P ; Human . Read more (PubMed: 27347123) »
  • Kumar P  et al. Differential Effects of Sodium Butyrate and Lithium Chloride on Rhesus Monkey Trophoblast Differentiation. PLoS One 10:e0135089 (2015). Read more (PubMed: 26266541) »

See all 4 Publications for this product

客户评价及客户问答

We unfortunately cannot provide ab20147 and ab54217 as FITC conjugated antibodies because we do not custom conjugate the antibodies.

We however have the following anti CD90 FITC conjugated antibodies that may be of your interest

ab...

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Thank you for your email.

CD271: We have ab8877 which is fully tested with Sheep samples and is guaranteed to work.

CD34: ab963, ab8536, ab30375, ab30377 are the sheep specific antibodies.

CD11b: It seems ab75476, ab62817...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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Verified customer

提交于 May 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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