Anti-CD34抗体[QBEND / 10] (Biotin) (ab30375)

概述

  • 产品名称Anti-CD34抗体[QBEND / 10] (Biotin)
    参阅全部 CD34 一抗
  • 描述
    小鼠单克隆抗体[QBEND / 10] to CD34 (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Human, Cynomolgus Monkey, Rhesus monkey
    不与反应: Rat, Sheep, Cow, Dog
  • 免疫原

    Human endothelial cell membrane vesicles.

  • 常规说明Each test is 10ul therefore the antibody volume supplied is 500ul.


    This antibody binds to the Class II epitope of CD34

性能

应用

Our Abpromise guarantee covers the use of ab30375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. in 100µl.



ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • 组织特异性Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • 序列相似性Belongs to the CD34 family.
  • 发展阶段On early hematopoietic progenitor cells.
  • 翻译后修饰Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD34 antibody
    • CD34 antigen antibody
    • CD34 molecule antibody
    • CD34_HUMAN antibody
    • Cluster designation 34 antibody
    • Hematopoietic progenitor cell antigen CD34 antibody
    • HPCA1 antibody
    • Mucosialin antibody
    • OTTHUMP00000034733 antibody
    • OTTHUMP00000034734 antibody
    see all

Anti-CD34 antibody [QBEND / 10] (Biotin) 图像

  • ab30375 staining CD34 in human intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/50 in PBS/BSA and incubated with the sample for 12 hours at 4°C.

    See Abreview

Anti-CD34 antibody [QBEND / 10] (Biotin) (ab30375)参考文献

This product has been referenced in:
  • Schrimpf C  et al. Pericyte TIMP3 and ADAMTS1 modulate vascular stability after kidney injury. J Am Soc Nephrol 23:868-83 (2012). Human . Read more (PubMed: 22383695) »
  • Poblet E  et al. QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors. J Cutan Pathol 21:224-8 (1994). Read more (PubMed: 7525669) »

See all 2 Publications for this product

Product Wall

We unfortunately cannot provide ab20147 and ab54217 as FITC conjugated antibodies because we do not custom conjugate the antibodies.

We however have the following anti CD90 FITC conjugated antibodies that may be of your interest

ab...

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Thank you for your email.

CD271: We have ab8877 which is fully tested with Sheep samples and is guaranteed to work.

CD34: ab963, ab8536, ab30375, ab30377 are the sheep specific antibodies.

CD11b: It seems ab75476, ab62817...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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提交于 May 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"